Detection of Oral Candidiasis Through the Microscope

Under the micsroscope, Candida albicans is yeast like structure that could be mistaken as oil droplets or fat globules. Candidiasis, commonly called yeast infection or thrush, is a fungal infection of any of the Candida species, of which Candida albicans is the most common. Candidiasis is the second most common cause of vaginal irritation, or vaginitis, and can also occur on the male genitals, particularly in uncircumcised men. In immunocompromised patients, the Candida infection can involve the esophagus and can become systemic, causing a much more serious condition: fungemia. Children, mostly between the ages of 3 and 9 years, can be affected by chronic mouth yeast infections, normally seen around the mouth as white patches. However, this is not a common condition. Yeast organisms are always present in all people, but are usually prevented from overgrowth by naturally occurring microorganisms. At least three quarters of all women will experience candidiasis at some point in their lives. The Candida albicans organism is found in the vaginas of almost all women and normally causes no problems.

However, when it gets out of balance with the other normal flora, such as lactobacilli, an overgrowth of yeast can result in noticeable symptoms. Pregnancy, the use of oral contraceptives, engaging in vaginal sex after anal sex in an unhygienic manner, and using lubricants containing glycerine have been found to be causally related to yeast infections. Diabetes mellitus and the use of antibiotics are also linked to an increased incidence of yeast infections. Candidiasis can be sexually transmitted between partners. Diet has been found to be the cause in some animals. Hormone Replacement Therapy and Infertility Treatment may be factors. Symptoms include severe itching, burning, and soreness, irritation of the vagina and/or vulva, and a whitish or whitish-gray discharge, often with a curdlike appearance. Many women mistake the symptoms of the more common bacterial vaginosis for a yeast infection. In a 2002 study published in the Journal of Obstetrics and Gynecology, only 33 percent of women who were self treating for a yeast infection actually had a yeast infection. Instead they had either bacterial vaginosis or a mixed-type infection. In men, symptoms include red patchy sores near the head of the penis or on the foreskin. The sores may feel irritated and itchy, and sometimes they will burn as well. Medical professionals use two primary methods to diagnose yeast infections: microscopic examination, and culturing. For the microscope method, a scraping or swab of the affected area is placed on a microscope slide. A single drop of 10% potassium hydroxide solution is then also placed on the slide. The KOH dissolves the skin cells but leaves the Candida untouched, so that when the slide is viewed under a microscope, the hyphae and pseudo spores of Candida are visible. Their presence in large numbers strongly suggests a yeast infection. For the culturing method, a sterile swab is rubbed on the infected skin surface. The swab is then rubbed across a culture medium. The medium is incubated for several days, during which time colonies of yeast and/or bacteria develop. The characteristics of the colonies provide a presumptive diagnosis of the organism causing symptoms. It is important to consider that Candida is part of the human body’s normal oral and intestinal flora and an infection is more a bloom than the work of a parasite, as in the case of some bacterial infections. Despite this, Candidiasis is sometimes misdiagnosed by medical personnel as bacterial in nature, and treated with antibiotics. This can lead to eliminating the yeast’s natural competitors for resources, and increase the severity of the condition. Candidiasis can allegedly be successfully treated either with home remedies or, in the case of a more severe infection, with either over-the-counter substances or prescription antifungal medications. Home remedies for candidiasis include the consumption or direct application of yogurt, probiotics, acidophilus tablets or salves, and even lightly crushed cloves of garlic, which yield allicin, an antifungal agent. Boric acid has also been used to treat yeast infections. Eating a diet consisting primarily of green, fresh, raw vegetables also may give relief.